Hi, I think the experiment is doable, but how would you decouple protein-protein interaction from folding of the unfolded protein due to protein interaction?
Reza Reza Khayat, PhD Assistant Professor The City College of New York Department of Chemistry, MR-1135 160 Convent Avenue New York, NY 10031 Tel. (212) 650-6070 ---- Original message ---- >Date: Fri, 14 Mar 2014 18:07:48 +0530 >From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> (on behalf of Anita P <crystals...@gmail.com>) >Subject: [ccp4bb] ITC with unfolded proteins >To: CCP4BB@JISCMAIL.AC.UK > > Hello everyone, > I have a query for the scientists working on > protein-protein interaction. > It is known that some proteins exist in unfolded or > molten globule state and attain structure on > interaction with other folded proteins. > Many a times, it is difficult to obtain the > structure of these complexes. > Is it possible to quantitatively determine the > thermodynamics of interaction between an unfolded > protein and a folded protein using ITC? Later may be > perform an alascan to determine the residues of the > unfolded partner involved in the interaction. > Please share your ideas > cheers** > Anita
