Jackie, We grow cells routinely and freeze pellets after fermentation. In general, proteins are fairly stable until you break cells so you are probably ok unless your protein is very heat labile and it sat at room temperature for hours. However as I mentioned, there is a kind of buffering from the cell that can stabilize the protein until you are ready to use it. The glycerol stocks are probably ok as well since you need just a small inoculum to get your culture growing. The plasmids may be the most affected, but you can sequence if you have aberrant expression.
Best, David -- David L. Blum, Ph.D. Director, Bioexpression and Fermentation Facility Department of Biochemistry and Molecular Biology University of Georgia 120 Green Street room A414A Athens, GA 30602 http://bff.uga.edu/ b...@uga.edu On Fri, Apr 25, 2014 at 12:43 PM, Jacqueline Vitali <jackie.vit...@gmail.com > wrote: > Dear colleagues, > > I know this is not related to ccp4 but I am in need of an answer and many > of you work with cells etc. > > My building had a major malfunction of electricity and the power backup > did not kick in. My -80C freezer was without power for over 24 hours until > I found out. Because it is small, it goes fast to room temp. I had many > glycerol stocks in it with cells, cells with plasmids etc. as well as cell > pellets. I am trying to rescue things. > > My question is what happens to cell pellets. I had many as I like to > start purification at the cell pellet level. Are these destroyed when they > go to room temp for 24 hours or are they ok? > > Thanks. > > Jackie Vitali > Cleveland State University > > > > >
