Hello All,

  I apologize for the non-crystal related question. I am trying to get a fully 
metal-free apo enzyme. The 6x His construct is consistently purified with some 
metal (20-30%). I have attempted chelating away the metal with up to 30 mM EDTA 
and DFO and then dialyzing it away, but this has shown little to no effect. Any 
thoughts or recommendations would be greatly appreciated. Thanks.

Adam

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