The Hoelz laboratory at the California Institute of Technology seeks to recruit an outstanding postdoctoral scientist in structural biology. Our laboratory focuses on the structure-function relationships of multi-protein macromolecular assemblies. Major research themes are (1) the nuclear pore complex and evolutionarily-related vesicle coats, and (2) histone modifying enzymes and their resident multi-protein complexes.
This position requires a recent Ph.D. degree in biochemistry/biophysics with a strong background in x-ray crystallography. The successful applicant should be a highly motivated individual with good organizational, communication, and interpersonal skills, who is able to carry out a structure determination project from the development of recombinant protein expression systems to structure refinement. The laboratory is newly constructed, well-funded, and equipped with modern biophysical instrumentation and current crystallization robotics. Importantly, Caltech has an exceptional infrastructure for macromolecular crystallography, including 40 % access to a high intensity, completely automated, state-of-the-art crystallography beamline equipped with a PILATUS detector at the Stanford Synchrotron Radiation Lightsource (SSRL). The position is available immediately and funded for 2+ years. Depending on performance, the position can be extended for up to five years. To apply, please send your CV, a statement of research interests, and the names and contact information of at least two references to André Hoelz (ho...@caltech.edu). Selected Publications Nuclear Pore Complex and Vesicle Coats Stuwe, T.S., Lin, D. H., Collins, L.M., Hurt, E., Hoelz, A.* (2014). Evidence for an evolutionary relationship between the large adaptor nucleoporin Nup192 and karyopherins, Proc. Natl. Acad. Sci. USA 111, 2530-2535. Lin, D.H., Zimmermann, S., Stuwe, T.S., Stuwe, E., Hoelz, A.* (2013). Structural and Functional Analysis of the C-terminal Domain of Nup358/RanBP2, J. Mol. Biol. 425, 1318-1329. [COVER] Stuwe, T.S., Schada von Borzyskowski, L., Davenport, A.M., Hoelz, A. (2012). Molecular Basis for the Anchoring of Proto-Oncoprotein Nup98 to the Cytoplasmic Face of the Nuclear Pore Complex, J. Mol. Biol. 419, 330-346. [COVER] Yoshida, K., Seo, H.S., Debler, E.W., Blobel, G., Hoelz, A. (2011). Structural and functional analysis of an essential heterotrimer on the cytoplasmic face of the nuclear pore complex, Proc. Natl. Acad. Sci. USA 108, 16571-16576. Hsia, K.C., Hoelz, A. (2010). Crystal structure of α-COP in complex with ε-COP provides insight into the architecture of the COPI vesicular coat. Proc. Natl. Acad. Sci. USA 107, 11271-11276. Ren, Y., Seo, H.S., Blobel, G., Hoelz, A. (2010). Structural and functional analysis of the interaction between the nucleoporin Nup98 and the mRNA export factor Rae1. Proc. Natl. Acad. Sci. USA 107, 10406-10411. Nagy, V., Hsia, K.C., Debler, E.W., Kampmann, M., Davenport, A.M., Blobel, G., Hoelz, A. (2009). Structure of a trimeric nucleoporin complex reveals alternate oligomerization states. Proc. Natl. Acad. Sci. USA 106, 17693-17698. Napetschnig, J., Kassube, S.A., Debler, E.W., Wong, R.W., Blobel, G. and Hoelz, A. (2009). Structural and functional analysis of the interaction between the nucleoporin Nup214 and the DEAD-box helicase Ddx19. Proc. Natl. Acad. Sci. USA 106, 3089-3094. Debler, E.W., Ma, Y., Seo, H.S., Hsia, K.C., Noriega, T.R., Blobel, G., Hoelz, A. (2008). A fence-like coat for the nuclear pore membrane. Mol. Cell 32, 815-826. [COVER] Hsia, K.C., Stavropoulos, P., Blobel, G., Hoelz, A. (2007) Architecture of a coat for the nuclear pore membrane. Cell 131, 1313-1326. Melcak, I., Hoelz, A.*, Blobel, G.* (2007). Structure of Nup58/45 suggests flexible nuclear pore diameter by intermolecular sliding. Science 315, 1729-1732. Enzymes Davenport, A.M., Collins, L.N., Chiu, H., Minor, P.J., Sternberg, P.W., Hoelz, A.* (2014). Structural and Functional Characterization of the α-tubulin acetyltransferase MEC-17 J. Mol. Biol. (epub ahead of print). Davenport, A.M., Huber, F.M., Hoelz, A.* (2013). Structural and Functional Analysis of Human SIRT1, J. Mol. Biol. 425, 507-509. [COVER] Stavropoulos, P., Nagy, V., Blobel G., Hoelz A. (2008). Molecular basis for the autoregulation of the protein acetyl transferase Rtt109. Proc. Natl. Acad. Sci. USA 105, 12236-12241. Stavropoulos, P., Blobel, G., Hoelz, A. (2006). Crystal structure and mechanism of human lysine-specific demethylase-1. Nat. Struct. Mol. Biol. 13, 626-632.*
