Hi Meisam, Here is what I have done before:
1. transfer the whole drop to a capillary and close both sides with plasticine. Notice that I am talking about the whole drop, not individual crystals. It is a lot simpler this way and involves less crystal manipulation. Make sure you remove any skin if present. 2. Transfer the well solution to an eppendorf tube. Don't make a new one, take the equilibrated solution from you tray. 3. Pack everything inside a styrofoam box with blue ice equilibrated in you incubator, as someone suggested. 4. When you get to the facility, reassemble your drop. Hopefully your crystals will survive the transfer (test before). Cheers, Mario Sanches On Wed, Jul 2, 2014 at 1:17 PM, Meisam Nosrati <meisam.nosr...@gmail.com> wrote: > Dear CCP4ers > > I need to transfer some crystals mainly in sitting drops to the site of > data collection without freezing them. > > I do not know what is the best solution to secure the boxes in their place > to minimize the disturbance. > > I am using the 24 well VDX plates with 10-80 microliter sitting drops. I > have one or two hanging drop boxes as well with 10 microliter size drops. > > If you have any experience about this matter, I greatly appreciate, if you > share it with me. > > Thanks > > Meisam Nosrati > -- Mario Sanches http://ca.linkedin.com/in/mariosanches
