Doing Fluroscence scan for protein crystal at Cu wavelength prior MAD or SAD experiment shows usually fig like this
https://www.dropbox.com/s/800463dlcmws6d4/fig.png 1- Fig A should represent X ray fluorescence scan. is it the same as X ray absorption scan. what does count represent in fig A (is it equal to the intensity of the emission). 2- i am wondering whether F double prime in Fig B is just a fit for counts in Fig A, it looks the same. could F" or counts be converted to I/I0 or F/I0 to replot the data against these value as in XANES. in other word, Is X ray fluorescence scan in fig A the same as X-ray absorption near-edge structure (XANES)? could XANES be collected at the same beamline where diffraction data are collected for crystal ? 3- could data from Fluorescence scan in Fig A or B used to determine the oxidation or coordination number for the metal say copper for example in protein? thank YOU regards T. DawoD
