Dear Careina, The pka of amino acids is not dependent on in vivo/in vitro, but on the local environment, e.g. free in solution vs. part of a folded protein. As part of a folded protein the shifts will be specific for the particular protein and I am not aware of any general rules. Best, Herman
Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Careina Edgooms Gesendet: Mittwoch, 13. August 2014 13:41 An: CCP4BB@JISCMAIL.AC.UK Betreff: [ccp4bb] off topic Sorry for off topic question, just wondering if anyone has come across a study that shows the residue pka of certain amino acids is different in vitro compared to in vivo? Best Careina
