Hi Manjalu, In my opinion, one way is to use iEX column. Depending of the Pi of you protein and the one of the BSA, if they are sufficiently different, Ion exchange is on possible way. To separate your protein from BSA, you have to give us more information about your protein if it's possible.
HTH. Nicolas ________________________________ De : CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] de la part de Manjula Ramu [manjula....@gmail.com] Envoyé : mercredi 8 octobre 2014 15:27 À : CCP4BB@JISCMAIL.AC.UK Objet : [ccp4bb] How to separate BSA present in Thrombin Hi all, My protein size is nearly the same size of BSA and it has His tag. If I have to cleave the tag I have to use Thrombin which is having huge amount of BSA in it. Now how will I separate this BSA from my protein. Thanks and Regards, Manjula R Research Scholar Department of Biophysics National Institute of Mental Health and Neurosciences Bengaluru-29, Karnataka INDIA E-mail: manjula....@gmail.com<mailto:manjula....@gmail.com> Mobile no:+91-9538553356 http://www.nimhans.kar.nic.in/ <https://www.nimhans.kar.nic.in/>
