Dear Herman, Assuming that the protein is eukaryotic, and glycans N-linked, various ways of dealing with the issue are described in PMID: 17355862.
Best wishes, radu ------------------------------------------ A. Radu Aricescu, PhD MRC Senior Research Fellow Associate Professor University of Oxford Wellcome Trust Centre for Human Genetics Division of Structural Biology Roosevelt Drive, Oxford OX3 7BN United Kingdom Phone: +44-1865-287564 Fax: +44-1865-287547 https://www.strubi.ox.ac.uk/research/a-radu-aricescu ---- Original message ---- >Date: Wed, 15 Oct 2014 13:03:13 +0000 >From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> (on behalf of >herman.schreu...@sanofi.com) >Subject: [ccp4bb] Trimming of carbohydrate chains >To: CCP4BB@JISCMAIL.AC.UK > >Dear Bulletin Board, > >I am struggling with a protein domain of 15 kDa, with about 22 kDa >carbohydrate attached. So far, the domain did not crystallize and I suspect >the carbohydrate may hinder crystallization. Completely removing the >carbohydrate results in low expression yields and poorly soluble protein, so I >would like to try to remove some, but not all carbohydrate. Does anyone has a >good protocol to trim, but not completely remove the carbohydrate? > >Thank you, >Herman Schreuder
