You have a gris 48 48 304 so need map 0 47 0 47 0 303 I guess. It should work I thought with asymm unit though Eleanor
On 20 October 2014 15:22, Scott Horowitz <horow...@umich.edu> wrote: > Hi all, > > Now that I have mapsig running, I am getting an error message that I am > confused by. The maps I am using are anomalous maps generated by FFT. > Originally, I had them covering just the asymmetric unit, but based on the > error message below, I then redid it where I covered a user defined extent, > where I went from 0 to the cell size for each axis, but the error message > stayed the same, which means perhaps I'm misunderstanding what it means. > Any help would be great, and the whole output is pasted below. > > Thanks, > Scott > > bash-3.2$ mapsig mapin H96LY10I01_14combfull.map mapin2 > H96LY10I01_6pt5combfull.map type ratio mapout test.map > <B><FONT COLOR="#FF0000"><!--SUMMARY_BEGIN--> > <pre> > > ############################################################### > ############################################################### > ############################################################### > ### CCP4 6.4: MAPSIG version 6.4 : ## > ############################################################### > User: horowsah Run date: 20/10/2014 Run time: 10:20:01 > > > Please reference: Collaborative Computational Project, Number 4. 1994. > "The CCP4 Suite: Programs for Protein Crystallography". Acta Cryst. D50, > 760-763. > as well as any specific reference in the program write-up. > > <!--SUMMARY_END--></FONT></B> > > > ############################################# > # TRANSLATION FUNCTION MAP SIGNAL ANALYSIS. # > ############################################# > > > BE WARNED THAT THIS PROGRAM ASSUMES PERIODIC MAP BOUNDARIES, > (I.E. THE LEFT-HAND EDGE ABUTS ONTO THE RIGHT-HAND EDGE) AND > THEREFORE MAY NOT LOCATE PEAKS AT THE EDGES OF THE MAP > CORRECTLY IN OTHER CASES. IN SUCH CASES THE MAP SHOULD BE > EXPANDED IN ONE OR MORE DIRECTIONS TO MAKE IT PERIODIC IN ALL > DIRECTIONS. > <B><FONT COLOR="#FF0000"><!--SUMMARY_BEGIN--> > <html> <!-- CCP4 HTML LOGFILE --> > <hr> > <!--SUMMARY_END--></FONT></B> > > Logical Name: MAPIN Filename: H96LY10I01_14combfull.map > > File name for input map file on unit 1 : H96LY10I01_14combfull.map > file size 1917228 ; logical name MAPIN > > > > Number of columns, rows, sections ............... 43 43 259 > Map mode ........................................ 2 > Start and stop points on columns, rows, sections 0 42 > 0 42 0 258 > Grid sampling on x, y, z ........................ 48 48 304 > Cell dimensions ................................. 42.7000 > 42.7000 258.2998 90.0000 90.0000 90.0000 > Fast, medium, slow axes ......................... Y X Z > Minimum density ................................. -0.04944 > Maximum density ................................. 0.19449 > Mean density .................................... -0.00000 > Rms deviation from mean density ................. 0.01025 > Space-group ..................................... 91 > Number of titles ................................ 1 > > > Labels: > [No title given] > > > > Translation modulus = 43 43 259 > *** ERROR - space group must be P1 if whole cell not given. > > > > -- > Scott Horowitz, Ph.D. > Research Associate > Howard Hughes Medical Institute > > University of Michigan > Department of Molecular, Cellular, and Developmental Biology > Bardwell lab > 830 N. University Ave, Room 4007 > Ann Arbor, MI 48109 > phone: 734-647-6683 > fax: 734-615-4226 >
