Hi Phhilippe,
T. Y. Teng,
J. Appl. Crystallogr.
23,
J. Appl. Crystallography 23:387–391 (1990)
Mounting of crystals for macromolecular crystallography in a
free-standing thin film
A method for mounting single crystals in macromolecular crystallographic
studies is described in which the crystal is suspended in a thin film.
The film is formed from a mixture of the crystallization buffer and a
hydrophilic viscous material, confined within a thin-wire loop by
surface tension. Compared with conventional crystal mounting methods,
this method greatly simplifies and speeds the mounting procedure, is
well suited to shock freezing and to optical monitoring of the crystals,
deforms fragile crystals less and gives a lower and more uniform
background in the X-ray diffraction patterns.
I do not have a copy, but if I remember well it was done with a copper
wire, hair and nylon loops came later.
Daniel
Le 03/11/2014 18:23, DUMAS Philippe (VIE) a écrit :
I am looking for the reference of this little paper (in J. Appl. Cryst ?)
describing how to make a loop and fish crystals...
You know, this kind of humble method used by all crystallographers (apart FEL
users).
Thank you in advance
Philippe Dumas
