Hi everyone, I have a few single chain antibodies (scFV) that I would like to express in a Fab format in bacteria for crystallization purposes.
Could you suggest some plasmids that have success in such kind of projects? Are there commercial plasmids consisting of antibody constant regions ready for bacterial expression, where I can just clone my scFv and it would be ready for Fab expression (probably periplasmic or secreted in supernatant) If commercial plasmids are not available, could you suggest where I could request them from? Thanks in advance, Ivan
