Dear CCP4BB Members, I'm trying to design a tRNA expression system for its co-crystal structure with an aminoacyl-tRNA synthetase (class Ia, eukaryotic). My question is how a specific tRNA is chosen among tRNAs of various anticodons for co-crystallization attempts. Can codon usage be a basis of the choice? How about levels of modification? Or do I have to try every anticodon to see which works?
Thank you in advance for your valuable advice, Hank
