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I'm a little confused. If you see from the density that a peptide flip is required, can't you just fix it? Or is it the case that the density is telling you that both conformations are present? In that case build alternative conformations and refine the occupancy. Dale Tronrud On 2/12/2015 9:07 AM, Kimberly Stanek wrote: > Hello all, > > I am in some of the final stages of refinement of a 1.5 A > resolution oligomeric protein and I'm noticing what appears to be > extra density corresponding to a carbonyl peptide flip near the > N-terminus of one of the chains. I was wondering if anyone had any > experience with this before and if so, could point me to any > relevant literature or references. > > Thanks, Kim > > -- Kimberly Stanek Graduate Student Mura Lab Department of > Chemistry University of Virginia (434) 924-7979 -----BEGIN PGP SIGNATURE----- Version: GnuPG v2.0.22 (MingW32) iEYEARECAAYFAlTdCpQACgkQU5C0gGfAG13+AACgmzI4srRRK51d/dTUTYCggNK5 EtUAoIkMS5lqN3+wrM3RVyPL2plQPjc9 =/wOR -----END PGP SIGNATURE-----
