Dear Fang Lu, These spots are from a small molecule crystal. There is one very short axial length (large distance between reciprocal lattice rows) and one quite long axis (the spots are close together in the straight reciprocal lattice row near the bottom of your diffraction pattern) You can measure these distances and come up with an approximate unit cell for the 2 dimensions from this one pattern. It is certainly not a protein.
Michael James On Wed, Feb 18, 2015 at 5:15 AM, Fang Lu <fangluwork...@gmail.com> wrote: > Hi all, > > I got this odd diffraction. Not sure what it is, detergent or protein? Any > ideas? > > The crystallisation condition is 2.4M Sodium Malonate. > GF buffer contains HEGA-10,Tris, KAcetate, MgAcetate, EDTA and DTT. > Protein size is around 100kDa. > > Thank you for your time. > > Fang > > [image: 内嵌图片 1] > >
