Hi I have a protein with two substrate. when I am doing the binding studies with the two substrate separately, I am finding one of the substrate to have similar kd and Km. however the km and kd values are almost 30 fold different for the other substrate. it binds 30 fold more tightly then you can think based on Km. I don't know how to explain this. Km and Kd can be different but does any one have seen that much difference in Kd and Km?
Thank you Dhiraj
