Hi All, I solved a structure using molecular replacement (Phaser) and the current R-factor and R-free is 0.2 and 0.25. The electron density looks great for residues and the ligand, however, the issue is with the B-values. The average B-value is 110 at this stage of refinement and I could not bring it down. All the residues are having a high B-values ranging from 90-120, while the R is good. I have tried several options in CCP4 (restrained refinement in Refmac) to bring it down and also used other MR programs, but still it still continues to be in the same range.
I will look forward to any suggestions. Thank you.
