On 04/01/15 11:23, Roger Rowlett wrote:
There are 8 possible space groups in the P422 family. Did you search
in all of these?
What he said. The answer should be "YES". Phaser is set up to do this
easily.
How many search models are likely to fit in the unit cell based on
Matthews analysis? Then there are questions about model selection and
preparation. How identical is the search model to the target? Does it
make sense to search for the domains separately?
If the domains are separate enough to be called "domains", it might be
worthwhile to try this.
Did you truncate the model side chains?
I usually try with the full sequence, and with a poly-Ala truncation.
Especially if the sequence identity between model and target is low.
BTW, you didn't mention anything about the similarity or identity of the
model. That's important in figuring out whether you should have been
successful or not. Much more so than the resolution of the data.
There are lots of ways to skin the cat depending on the problems
encountered. 3.0 A may be challenging for a low identity search model.
__________________
Roger Rowlett
Gordon & Dorothy Kline Professor
Department of Chemistry
Colgate University
On Apr 1, 2015 11:06 AM, "Sekharreddy M" <vijayasekk...@gmail.com
<mailto:vijayasekk...@gmail.com>> wrote:
HI All,
I am trying to find MR solution for one of the protein
crystals with 3.0A resolution, but not able find solution using
Phaser (PHENIX).The protein has two domains.and the probable best
space group identified was p4212(HKL2000).
I am novice in using crystallization tools for solving
structures.I am stuck at this stage and I appreciate any help in
this regard.
Thank you.
--
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All Things Serve the Beam
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David J. Schuller
modern man in a post-modern world
MacCHESS, Cornell University
schul...@cornell.edu
