-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Dear Xiao,
you would probably get started with molecular replacement to place one domain after the other. Once you solved the structure you can use external restraints to the known structures. If your resolution is decent and the domains are not disordered, you can remove the external restraints as you progress. The names vary, but all refinement programs I am aware of can make use of external restraints. Best, Tim On 05/12/2015 07:56 AM, Victor Xiao wrote: > Dear all, > > I am trying to model a full-length double-domain protein in dimer. > The structure of both N and C terminal domains are known and the > linker region of 5 residues are present in both structure (the > linker might be flexible though). The N-terminal domain's structure > is also a dimer, which can be used as another restraint. > > Does anyone know any good tool for modeling such full-length dimer? > Thanks in advance. > > Best, > > Xiao > - -- - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen phone: +49 (0)551 39 22149 GPG Key ID = A46BEE1A -----BEGIN PGP SIGNATURE----- Version: GnuPG v1 iD8DBQFVUaRgUxlJ7aRr7hoRAi/AAJ9rQfJZtY3GCbyX0SQcK/rEMIWIDgCeN7kl RqL/+siVw7a3E1A29Ajj5uU= =TtOp -----END PGP SIGNATURE-----
