Hi fabulous cc4bbs, I had recently asked you about using HPLC or FPLC for protein purification.Now I have another question relating to that. I am trying to buy a new SEC column for the FPLC for protein purification but am getting some resistance from some experienced people in the lab who have now moved from promoting HPLC to sephadex beads instead of superdex column.
Could you please suggest what is the major difference between sephadex columns and superdex columns in your personal experience? Are there any sephadex columns or just beads? I have always used superdex GE prepacked columns which have been fabulous for Gel filtration. Please advise and as always thanks in advance. ivan
