Hi Eric, What does your map look like? Do you see features that don't come from the search model? That's the key. That said, with a TFZ of above 10, I'd be rather positive about my prospects.
Andreas On Tue, May 19, 2015 at 2:36 AM, Eric Karg < 0000052044071b36-dmarc-requ...@jiscmail.ac.uk> wrote: > Hi all, > > Running Phaser using the apo protein as search model on a ~2.5 A dataset > of a protein-DNA complex, I get a single solution but with low RFZ. The map > looks reasonable but I was wondering why the RFZ is so low. Would this > solution be acceptable? > > SOLU SET RFZ=3.2 TFZ=8.4 PAK=0 LLG=66 TFZ==10.6 RFZ=2.9 TFZ=13.7 PAK=0 > LLG=203 > TFZ==14.0 LLG=1440 TFZ==34.2 > SOLU SPAC P 62 2 2 > SOLU 6DIM ENSE ensemble1 EULER 181.8 55.7 74.8 FRAC 0.27 0.26 -0.40 > BFAC -7.38 > SOLU 6DIM ENSE ensemble1 EULER 4.5 120.2 7.9 FRAC -0.31 0.20 -0.09 BFAC > 12.61 > Ensemble ensemble1 RMS variance(s): 0.87 > > Thank you for your help! >
