Hi Weifei, We use SSC buffer for annealing oligos (we use DNA). That works pretty fine with us. Give a try. Composition is in below URL
http://www.bioprotocols.info/reagent_and_buffer_recipes/20x-SSC.php Good Luck, Venkatareddy. On Fri, Jul 3, 2015 at 8:44 PM, ChenWeiFei <weife...@outlook.com> wrote: > Dear all, > I want to get a complex of DNA-RNA-protein. But I have a big problem of > annealing DNA-RNA. > The length of DNA is 19nt and RNA is 17nt. > Annealing protocol: > 2uM DNA > 2uM RNA > 10mM Tris-Hcl > 100mMNaCl > 1mMEDTA > > Heated to 95 for 5min, cooling down slowly for nearly 2h to room > temperature. > > I can just get a result of two single strand DNA/RNA. PAGE analysis. > > No double helix was founded. > > Does anyone have the same problem or know how to fix it. > > Thank you for your answering. > > Best regards, > Weifei -- *Venkatareddy Dadireddy,B1-10,Prof. S. Ramakumar's Lab,Dept. of Physics,IISc, Banglore.Cell: 07259492227*
