Hi there, What about trying the ''olde'' method of a thin layer of oil around the crystal? No guarantee whatsoever that this will work but if you don't try it you won't know.
Fred. ________________________________________ From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Alejandro Madrigal Carrillo [amadri...@email.ifc.unam.mx] Sent: Monday, February 22, 2016 11:00 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] cryo for crystals with CTAB as precipitant Dear All, I got crystals using CTAB 10mM as precipitant, but I have had problems with the cryoprotectant solution which freezes. Has anybody collected crystallographic data with crystals grown in this detergent? What cryoprotectant do you recommend to test? Two papers report use of glycerol as cryoprotectant (Brown MA et al., 2005; glycerol 40% Ren A et al, 2010 ). But at -20oC the reservoir solution plus cryoprotectant freezes. I tried with many cryoprotectants (glycerol 40%, EG 35%, PEG 400 35%, hexanediol 50%, Sucrose 28%, Xylitol 30%, etc) but solution at -20oC looks cloudy or frozen. Any suggestion will be appreciated. Thanks in advance, Alejandro Madrigal Carrillo, M.Sc. Laboratorio de Biofísica de Biomacromoléculas, 205 sur Departamento de Bioquímica y Biología Estructural Instituto de Fisiología Celular Universidad Nacional Autónoma de México Circuito Exterior S/N. Ciudad Universitaria México, D.F., México, 04510 Teléfono: +52 55 5622 5640 ----- Upozornění: Není-li v této zprávě výslovně uvedeno jinak, má tato E-mailová zpráva nebo její přílohy pouze informativní charakter. Tato zpráva ani její přílohy v žádném ohledu Biotechnologický ústav AV ČR, v. v. i. k ničemu nezavazují. Text této zprávy nebo jejích příloh není návrhem na uzavření smlouvy, ani přijetím případného návrhu na uzavření smlouvy, ani jiným právním jednáním směřujícím k uzavření jakékoliv smlouvy a nezakládá předsmluvní odpovědnost Biotechnologického ústavu AV ČR, v. v. i. Disclaimer: If not expressly stated otherwise, this e-mail message (including any attached files) is intended purely for informational purposes and does not represent a binding agreement on the part of Institute of Biotechnology CAS. The text of this message and its attachments cannot be considered as a proposal to conclude a contract, nor the acceptance of a proposal to conclude a contract, nor any other legal act leading to concluding any contract; nor does it create any pre-contractual liability on the part of Institute of Biotechnology CAS
