Probably you can not use thiocyanate as cryoprotectant because its a chaotropic agent. Although its a very mild denaturant (destabilizer) but at high concentration, it can destroy your crystals. I have recently used 30 % sucrose for cryo protection of crystals growing under same condition and it worked beautifully. My crystals were cracking in glycerol.
Dhiraj On Sep 27, 2016, at 5:43 PM, Alex Lee <alexlee198...@gmail.com<mailto:alexlee198...@gmail.com>> wrote: Hi CCP4bb members, I have a condition for my protein crystals at "0.2M sodium thiocyanate, 25% PEG3350, pH6.9". I'd like to try cryoprotectant with just higher concentration of sodium thiocyanate, but I have no google hits of the range of concentration I should use. I wonder anyone in this forum tried to use sodium thiocyanate as cryoprotectant? What's the concentration range for this if used as cryoprotectant? Thanks.
