Hi Alex (1) Go to “Settings -> Experiment settings -> Detector” and change the value there; you should probably re-run integration, though since your value is only just outside the range of 0.7 - 1.3, I might not worry too much about that, and I don’t think it would make a big difference.
(2) Don’t worry about this too much if it only happens for two images. Have a look at the offending images, and if (a) there is anything _obviously_ different about them compared to the preceding and following images, you _could_ omit them from the scaling (I wouldn’t bother to re-run the integration). (b) they look broadly similar to the preceding and following images, I wouldn’t worry at all. (c) if this happened for a large proportion of the images I would be more concerned (which leaves the question, “what is a large proportion”?). (3) My view is that most of the “warnings” in Mosflm are advice that can be used to improve data processing; you will learn which are more important through the lens of experience. (4) I’d also work through the online tutorial which will give you a pretty well comprehensive introduction to everything there is to know about changing parameters etc in iMosflm. It takes anywhere from 1 to 4 hours to work through, depending on how carefully you do it. HTH Harry -- Dr Harry Powell Chairman of International Union of Crystallography Commission on Crystallographic Computing Chairman of European Crystallographic Association SIG9 (Crystallographic Computing) > On 3 Dec 2016, at 21:48, Alex Lee <alexlee198...@gmail.com> wrote: > > Dear CCP4bb members, > > I am new to iMosflm data processing (I use HKL2000 but I really want to learn > iMosflm). After automatic index, cell refinement and integration in iMosflm, > I got 2 warnings. > > 1. Error in detector gain. BGRATIO of my data lies outside the 0.7 to 1.3 > range (mine is 0.64) Imosflm suggests changing GAIN to 0.14. How do I change > GAIN to the 0.14 value? Do I need to change GAIN before integration? > > 2. Poor standard profiles in some areas. Both Image 1 and Image 13 have some > boxes with cc lower than 0.5. Should I exclude these two frames? How to do > it? I know in HKL2000 after scaling there is "exclude frames" option, but I > could not find it in iMosflm. > > Thanks. > >
