Dear Jacob, You did not miss the 101 class, it is just too much to remember.
E. coli metabolome when growing on glucose (Lehninger, 2013): http://www.fullonline.org/science/ecoli_metabolome.png The book does not provide explanation for this, but I remember reading somewhere else that glutamate is a metabolic intermediate that mediates osmotic regulation. When growing in high osmotic environments, E. coli accumulates glutamate to match the outside osmotic pressure. Hopefully this is not incorrect, because that's what I've been telling my students. Regards, Napo ----- Mensagem original ----- De: "Jacob Keller" <kell...@janelia.hhmi.org> Para: CCP4BB@JISCMAIL.AC.UK Enviadas: Quarta-feira, 11 de Janeiro de 2017 22:45:03 Assunto: [ccp4bb] Completely Off-Topic Dear Crystallographers, Was anyone else aware that in E coli the intracellular glutamate concentration is ~100 mM? Also other cell types (yeast, mammalian) are 10s mM. Anything to say about this? I learned of this just recently, and have been amazed about it for more than a week. Did I miss this in Biochem 101? Does it matter? JPK ******************************************* Jacob Pearson Keller, PhD Research Scientist HHMI Janelia Research Campus / Looger lab Phone: (571)209-4000 x3159 Email: kell...@janelia.hhmi.org *******************************************
