Hi, You can find GroESL expressing plasmid and plasmids with different chaperones combinations from prof. Bernd Bukau lab on Addgene:
https://www.addgene.org/Bernd_Bukau/ Best Michal 2017-01-20 3:19 GMT+01:00 Patrick Loll <pat.l...@drexel.edu>: > Hello everyone, > > Please allow me to kill two off-topic birds with one email ‘stone’: > > 1) Can anyone suggest where I can lay hands on the pREP4-GroESL plasmid > for bacterial co-expression of GroEL and GroES? I’ve checked with the > original author (Philip Cole), but it’s been decades since he published on > this, and he’s not sure he can find the plasmid. However, I see recurrent > (and recent) references to the plasmid in the literature, so I guess that > it’s loose in the wild… > > 2) We’re looking for a contract research organization to do some Bac-Mam > expression for us (we’re in a hurry to get some results, so we’d like to > farm it out rather than get the technology going in-house). Can anyone > recommend a group (ideally in the US, for logistical ease)? > > Thanks for any help. > > Cheers, > > Pat > > > > ------------------------------------------------------------ > --------------------------- > > Patrick J. Loll, Ph. D. > > Professor of Biochemistry & Molecular Biology > > Director, Biochemistry Graduate Program > > Drexel University College of Medicine > > Room 10-102 New College Building > > 245 N. 15th St., Mailstop 497 > > Philadelphia, PA 19102-1192 USA > > > (215) 762-7706 > > pjl...@gmail.com > > pl...@drexelmed.edu > >
