Dear all: I would like to seek your suggestion on protein crystallization from phase separation. We recently observed many small round droplets shown in our protein/DNA crystallization. Condition are 0.8M-1.6M LiSO4; 20mM MgCl2; pH 5-8; protein conc. ~15mg/ml). The UV microscope confirms those are protein-rich phase separation. We have tried to change conc. of LiSO4 and pH. Still we got different size and amount of small round droplets. At 20 degree, those droplets appear within one day and at 4 degree, it takes two-three days. We also tried additive and silver bullet screen. So far, we have not found a condition to have protein crystals. The protein is already truncated. Several DNA constructs are on-going. At this point, I would like to seek your advice on the method to optimize the condition. Based on
PS. Any people have luck with protein crystallization by streaking the Gelationous protein to new drop as shown in http://xray.bmc.uu.se/terese/tutorial3.html . Can you please share your experience with us? Thanks for your help. Joseph Ho
