Hi, I have an EM map of a tetrameric protein. It was painful to work with this map since it is in P1 spacegroup, although 4-fold symmetry was already applied during map reconstruction.
I noticed that people used MAPMAN to transform spacegroup, however, it seems not working for me. The map remained in P1 spacegroup afterwards. I used mtz file converted from .mrc and the tetrameric protein model as input and choose "run fft to generate simple map". I also specified "output map in ccp4 format to cover all atoms in pdb". In "infrequently used options", I input P4 in "generate map in spacegroup". Everything else was left as default. Any suggestions will be appreciated. Thanks! QC
