Fab fragment binding towards long loop
在2017年07月04日 23:22，dongxiaofei 写道：
Dear ALL,
I want to make a protein crystal,but there is a long loop between domains of
protein , which contains two small domains owning about 40 amino acids
respectively and a loop about 70 amino acids.
Loop is so long and flexible ,but I don't want to delete some fragments,because
it may be important for protein's function of a histone demethylase.
Besides, the surface charge of protein is whole negative .
I have tried a long time but it is hard to me to get crystal.
Would be very grateful for any advice!
Thanks
Dong Xiao
