Dear all, My apologies for not being able to see the pictures. I have attached them as PDFs here but not sure if you will be able to get the files.
Anyway, many thanks to all who have reply via ccp4bb or privately with suggestions. Indeed the protein is purified from the natural source so there are numerous possibilities. Kind regards, Isabel On 7 Jul 2017, at 12:57, Dr. Isabel De Moraes <isabel.de-mor...@diamond.ac.uk<mailto:isabel.de-mor...@diamond.ac.uk>> wrote: Any suggestions regarding to the positive densities? The crystallisation condition only has NaCl and CaCl [cid:DD08D41F-CAB5-458D-BBD0-5DDCADC746FF@diamond.ac.uk] [cid:B6BDEE5E-325C-4FEF-A7BC-B4F679D5315F@diamond.ac.uk] Best regards, Isabel -- This e-mail and any attachments may contain confidential, copyright and or privileged material, and are for the use of the intended addressee only. If you are not the intended addressee or an authorised recipient of the addressee please notify us of receipt by returning the e-mail and do not use, copy, retain, distribute or disclose the information in or attached to the e-mail. Any opinions expressed within this e-mail are those of the individual and not necessarily of Diamond Light Source Ltd. Diamond Light Source Ltd. cannot guarantee that this e-mail or any attachments are free from viruses and we cannot accept liability for any damage which you may sustain as a result of software viruses which may be transmitted in or with the message. Diamond Light Source Limited (company no. 4375679). Registered in England and Wales with its registered office at Diamond House, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom --------------------------------------------------------- Dr Isabel Moraes Membrane Protein Laboratory Group Leader Diamond Light Source Ltd, Harwell Science and Innovation Campus, Oxfordshire, OX11 ODE, UK --------------------------------------------------------- [cid:b2df3ed8-aa8e-4a06-a691-8732bdbf96ce@fed.cclrc.ac.uk]