Hi Narayanan, This doesn't address your question; may be you could go-around this problem by using a different purification tag., say like GST?!!
Good Luck, Partha On Fri, Sep 15, 2017 at 7:54 AM Narayanan Ramasubbu < [email protected]> wrote: > Hi. We are working on a periplasmic protein that breaks naked glycans in > peptidoglycans. There is truncated structure available but our target is > the full length protein. The difficulty us that it strongly binds to the > resin with or without his.tag. Changing the resin to acrylamide did not > help. > Has anyone come across similar problem and how was it resolved. > The pdb structure is the catalytic domain and mussing a region that, in my > opinion, binds to the resin. > Thank you in advance > Sent from my iPhone
