Hello everyone,
I wonder if there is a consensus for what is currently the best system to
express transmembrane proteins in mammalian cells?
I think that baculovirus transduction ("Bacmam" anf the likes) has been
used historically more (?) but would like to know if the modern adenovirus
systems offer any advantages in terms of expression levels. I used both in
the past but not for transmembrane proteins and never compared them back to
back for the same protein.
Has anyone attempted a direct comparison? (It has to be mammalian cells, so
baculovirus/insect cells won't do).
Thanks for any comments/insights,
Nate
