Dear All, I have a protein from which I'd like to lose the co-purifying ligand (Coenzyme A). The protein is his-tagged. The plan here is to bind it to a resin and then run over a mild denaturant solution to encourage ligand loss (it seems that extended dialysis does not achieve this).
Our starting protocol might aim at using roughly 1-1.5M Urea. If anyone has trialled this (or a different protocol, say extremely high NaCl) for a similar or different protein:ligand combination then I'd be keen to hear your thoughts. Thanks, Andy
