That map does not look like a 5A map? I guess you mean something else.. Eleanor
On 3 November 2017 at 11:00, <herman.schreu...@sanofi.com> wrote: > You are right. In this case, I would put some waters in it, refine and see > if the density gets any clearer. However, since from this perspective the > density is quite far away from the protein, it could be a very disordered > PEG, which, even at high resolution, might be impossible to fit. > > > > Best, > > Herman > > > > *Von:* Abhishek Anan [mailto:rendezvous.a...@gmail.com] > *Gesendet:* Freitag, 3. November 2017 11:10 > *An:* Schreuder, Herman /DE > *Cc:* CCP4BB@jiscmail.ac.uk > *Betreff:* [EXTERNAL] Re: [ccp4bb] another unknown density problem > > > > Dear Prof Schreuder > > Here are another couple of perspectives from coot. The density is too far > and isolated from the peptide chain to be an alternate conformation or > conformational change. The density of the peptide chain does not look good > because it was truncated at 5A for clarity. > > Best regards > > Abhishek > > > > On Fri, Nov 3, 2017 at 9:33 AM, <herman.schreu...@sanofi.com> wrote: > > Dear Abhishek, > > > > To me, it looks like an alternative conformation of the peptide chain or > maybe even a conformational change with respect to the starting model. The > peptide chain does not look too well defined, despite high resolution > electron density. > > > > Best, > > Herman > > > > *Von:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *Im Auftrag von > *Abhishek Anan > *Gesendet:* Freitag, 3. November 2017 09:26 > *An:* CCP4BB@JISCMAIL.AC.UK > *Betreff:* [EXTERNAL] [ccp4bb] another unknown density problem > > > > Hi all, > > I have an "unknown" density in the map. I have tried to fit it to PEG but > it doesn't fit very well. I was wondering if there are other PEG-related or > other molecules I could try. > > The crystal grew in TRIS-HCl and PEG MME 2K. > > Thank you > > Abhishek > > >
