Hello everyone! In which condition can we add ligand/substrate directly to the expression medium for overexpression protein? One protein of i crystallized is a enzyme that catalyze sterols substrate, as we know the solubility of sterols is very poor, and the Km of the enzyme towards sterols around 0.5-1mM. Can i add sterols directly to the medium , when i expression my protein ? If not , then in which condition can we do like this, for inhibitor ? i remeber i read one paper did like this.
Sincerely! Liuqing chen
