Dear all,
I am working currently on a structure that, nicely, presents two different orientations between its domains when one compares monomers of the tetramer in the asymmetric unit.
I notice, in this nature gift, that a helix, probably central (in its role) for the relation (orientation) between the two domains, assumes different relation to other one (helix, that belongs to one of the domains) such that they are (significantly) more or less "coiled-coiled"one to another, once the domains are in the "close" or "open" conformation. We have already analyzed the hydrogen bonds and salt bridges that are disrupted (or formed) due to the different (domain and helix) conformations.
I wonder whether there is a metric (easy to evaluate) to characterize how much the two helix are "around each other" (id est, how much "coiled-coiled" they are) and, preferably, a software to calculate this metric.
Thank you, Jorge State University of Ponta Grossa ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
