BS”D Dear All,
I would just like to know how refmac handles the following setup. A deposited structure (2.8Å resolution) is a homotrimer protein (chains A, B,C) with bound dsDNA (chains D, E), and some water molecules. There is NCS (type: local) defined between the three protein chains (A to B, B to C, A to C). There is one TLS group defined for Chain A, and nothing else. But all the atoms (all chains) have ANISOU cards. So is Chain A being refined with TLS, and the other chains with classic anisotropic B’s (which is not justified at this resolution), or is something else happening? Thanks Harry -------------------------------------------------------------------- Harry M. Greenblatt Associate Staff Scientist Dept of Structural Biology harry.greenbl...@weizmann.ac.il<mailto:harry.greenbl...@weizmann.ac.il> Weizmann Institute of Science Phone: 972-8-934-6340 234 Herzl St. Facsimile: 972-8-934-3361 Rehovot, 7610001 Israel ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
