Ok, we just needed to renumber the reside and change HETATM to ATOM and the became linked to the main chain again.
Thanks all *Renato Mateus Domingos* *PhD student at Departamento de Genética e Biologia Evolutiva* *Universidade de São Paulo, Brazil* *Phone: (+55)(11)30917590* On Tue, Oct 16, 2018 at 5:03 PM Renato Mateus Domingos <rmdomin...@ib.usp.br> wrote: > Hi all > > I am trying to Mutate a Cys-SH to Cys-SO3. > > I was replacing CYS with a OCS, using Coot: > > 1) centre on the CYS of interest > 2 ) Extensions -> Modelling -> Replace Residue ... -> OCS -> Mutate > > However the residue appears disconnected from the main chain, and I could > not find a way to make the proper bonds. > > Anyone can help? > > Renato > *Renato Mateus Domingos* > *PhD student at Departamento de Genética e Biologia Evolutiva* > *Universidade de São Paulo, Brazil* > *Phone: (+55)(11)30917590* > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
