This is the first thing you need to do before going down the rabbit hole looking for a cryo protectant. Mitegen make it much easier then in the 'old days' of capillaries.
Good luck, Len lmtho...@ou.edu On Oct 20, 2018 2:02 AM, Andreas Förster <docandr...@gmail.com> wrote: Dear Firdous, get some Mitegen sleeves and test your crystals at RT. Before you establish diffraction, there’s no need to agonize about cryo-conditions. Good luck! Andreas On Fri, 19 Oct 2018 at 23:57, Firdous Tarique <kahkashantari...@gmail.com<mailto:kahkashantari...@gmail.com>> wrote: Dear members I have got beautiful crystal hits in SaltRx screens which are not diffracting to a good resoultion. All of them are salt based condition and I am not able to formulate a good cryoprotectant for these crystals. I also think that in my case the poor resolution is due to a poor cryoprotectant selection. The conditions are as follows: 1> 4M Ammonium Acetate 100mM Bis Tris Propane pH 7.0 2>0.5M KCN 100mM Tris pH8.5 3>1.5M LiSo4 100mM Bris Tris Propane pH 7.0 4>4M Sodium Nitrate 100mM Tris pH8.5 5>1.5M Sodium Nitrate 100mM Sodium acetate pH 4.6 There are few more conditions but so far not able to see good diffraction with using lower peg and glycerol based cryoprotectants. Can anybody suggest me good cryos conditions for salt based crystallization conditions or anything good for SaltRx crystallization hits. Thanks Firdous ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwMFaQ&c=qKdtBuuu6dQK9MsRUVJ2DPXW6oayO8fu4TfEHS8sGNk&r=LYFS_Pt-UDexWUsCj6bDwQ&m=-ToI6ga-FYygb9nKnP_gQXDS5-my58CypHjzkpTAcXc&s=aphz-daUOztDbG1vJsutT1p4M_P9ICmhAl2OkEdU63Q&e=> ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwMFaQ&c=qKdtBuuu6dQK9MsRUVJ2DPXW6oayO8fu4TfEHS8sGNk&r=LYFS_Pt-UDexWUsCj6bDwQ&m=-ToI6ga-FYygb9nKnP_gQXDS5-my58CypHjzkpTAcXc&s=aphz-daUOztDbG1vJsutT1p4M_P9ICmhAl2OkEdU63Q&e=> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
