Hi Joseph, Apologies for not answering your question really :-) But, unless you plan to focus on very peculiar insect cell proteins, I would suggest considering HEK cells. See PMID: 30455477 for one of several possible systems. Cheaper, easier, wave bag-friendly... Overall better than insect, especially for membrane and secreted proteins. All vectors are with Addgene, early passage cells from ATCC.
Best wishes, Radu -- Radu Aricescu MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge Biomedical Campus Cambridge CB2 0QH, U.K. tel: +44-(0)1223-267049 fax: +44-(0)1223-268305 www: http://www2.mrc-lmb.cam.ac.uk/group-leaders/a-to-g/radu-aricescu > Dear ccp4 community, > > > I'll soon start my own lab and together with other colleagues, would like to > establish a facility to do large scale insect cell culture for > crystallography. Has anyone had any experience with the wave bag systems for > this purpose (for instance from GE)? I have heard that culture infection can > be a problem with this system. > > > Any advice or other helpful tips in this regard would be much appreciated. > > > Best regards, > > Joseph. > > > > > Joseph S. Brock | PhD > Researcher > Drew Lab > Department of Biochemistry and Biophysics > Stockholm University > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
