Anomalous is the way to go but it helps to think about the final concentrations used..... With 50 mM PO4 (buffer) and 2 M  SO4 (ammonium sulfate) most "bindin sites in quesiton" will be occupied by SO4...

My 2 cents

Jeroen

On Sat, Feb 16, 2019 at 4:07 AM 张士军 <21620150150...@stu.xmu.edu.cn <mailto:21620150150...@stu.xmu.edu.cn>> wrote:

    Dear all

    I have got a crystal grown at the condition both have ion of SO4
    and PO4, and the diffraction resolution is very well, but the
    problem is coming: how to tell which is which just from electron
    density? I think they are exactly same. Thanks a lot !!!

    Beat Regards

    Shijun


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--
*Dr.math. et dis. nat.Jeroen R. Mesters*
Deputy, Lecturer, Program Coordinator /Infection Biology
/ <http://www.uni-luebeck.de/studium/studiengaenge/infection-biology/introduction.html>Visiting Professorship (South Bohemian University) in Biophysics
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*University of Lübeck*
Center for Structural and Cell Biology in Medicine
*Institute of Biochemistry*

Tel +49 451 3101 3105 (secretariate 3101)
Fax +49 451 3101 3104
jeroen.mest...@uni-luebeck.de <mailto:jeroen.mest...@uni-luebeck.de>
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