Dear all, Thanks for the suggestions about the analytical columns. I plan to pack some columns myself with the suggested beads and also I am keen to trying monolithic columns.
However, I don't know if anyone tried TSKgel columns for mega dalton protein complexes or viruses? Best wishes, Mohinder Pal ---------------------------------------------- "Whatever you’re meant to do, do it now. The conditions are always impossible.” Doris Lessing ---------------------------------------------- > On 18 Apr 2019, at 07:36, Bärbel Blaum <baerbel.bl...@intherabio.com> wrote: > > Hi Mohinder, > > if you are up to trying something new the monolithic columns by Biaseparation > (analytical or prep) are well suited for big molecules / particles and worked > very well for me. The fittings work with the GE chromatography instruments. > > https://www.biaseparations.com <https://www.biaseparations.com/> > > Good luck, > > Bärbel > > -- > Bärbel Blaum, PhD > Inthera Bioscience AG > Einsiedlerstrasse 34 > CH-8820 Waedenswil > Switzerland > E-Mail: baerbel.bl...@intherabio.com <mailto:baerbel.bl...@intherabio.com> > Phone: +41 43 477 94 72-- > > > > Von: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK > <mailto:CCP4BB@JISCMAIL.AC.UK>> im Auftrag von David Blum <dlb...@gmail.com > <mailto:dlb...@gmail.com>> > Antworten an: David Blum <dlb...@gmail.com <mailto:dlb...@gmail.com>> > Datum: Mittwoch, 17. April 2019 um 21:48 > An: <CCP4BB@JISCMAIL.AC.UK> > Betreff: Re: [ccp4bb] analytical gel filtration columns > > Mohinder, > > You could also try HPLC where you should get better results due to more > theoretical plates. One option would from Phenomenex. > 10E6A Phase Information > · Molecular Weight Range: 60 K - 10,000 K > https://www.phenomenex.com/Products/HPLCDetail/phenogel > <https://www.phenomenex.com/Products/HPLCDetail/phenogel> > > David L. Blum, Ph.D. > Bioexpression and Fermentation Facility | Director > Master of Biomanufacturing and Bioprocessing | Director > 120 Green Street | Life Sciences Bldg rm A414A | Athens, GA 30602 > 706-542-1035 <mailto:706-542-1035> | b...@uga.edu <mailto:b...@uga.edu> | > bff.uga.edu <http://bff.uga.edu/> | biomanufacturing.uga.edu > <http://biomanufacturing.uga.edu/> > > > > On Wed, Apr 17, 2019 at 11:11 AM Mohinder Pal <mohinder.o....@gmail.com > <mailto:mohinder.o....@gmail.com>> wrote: >> Dear all, >> >> I would like to gel filter a multi protein complex (1.4MDa) as the final >> purification step. I have tried tandem Superose 6 columns and this complex >> elutes close to void volume as it is a very elongated molecule. >> >> >> I was wondering if someone could suggest different analytical columns for >> better resolution as I plan to add more components to this existing complex. >> >> Best wishes, >> >> Mohinder Pal >> >> ---------------------------------------------- >> "Whatever you’re meant to do, do it now. The conditions are always >> impossible.” >> Doris Lessing >> ---------------------------------------------- >> >> >> >> >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >> <https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1> > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > <https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1> > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > <https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
