Bear in mind that if you have cold buffers going into a room temperature (or warmer) pumps, you can get outgasing that will cause inaccurate flow or loss of priming for the pumps. You may be able to work around by using freshly degassed eluents, but the outgasing will still be a problem for long runs.
On Fri, Jun 7, 2019 at 11:32 AM Gianluca Cioci <ci...@insa-toulouse.fr> wrote: > > Hi, > > a cheap solution would be to put buffers, fraction collector, and the column > (if not too big) > inside a cold cabinet, next to the FPLC. > you probably need to use longer pipes but it works > > Best regards, > > GIA > > > > Le 07/06/2019 16:51, - - a écrit : > > Dear all, > > our cold cabinet FPLC is rotting away due to the high humidity in our lab. > Anybody having a room temperature setup, where only the buffers, column(s) > and fractions are being cooled? If so, by which means (cooling spiral, water > pump, peltier element?). Thank you for your suggestions! Michael > > > ________________________________ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > > -- > 1st French Congress on Integrative Structural Biology > Please check: http://bsi-2019.ipbs.fr > > Dr. Gianluca CIOCI > BioCatalysis Team > http://www.lisbp.fr/en/research/catalysis-and-molecular-enzymatic-engineering--ead1.html > PICT - Plateforme Intégrée de Criblage de Toulouse > http://cribligand.ipbs.fr/index.html > > Tel: +33 (0)5 61 55 97 68 > Tel: +33 (0)5 61 55 94 91 > E-mail: ci...@insa-toulouse.fr > > LISBP - INSA de Toulouse > 135 avenue de Rangueil > 31077 Toulouse CEDEX 04 > www.lisbp.fr > > > ________________________________ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
