Hi James,
7) or 8) What are a few different collection methods
including at RT for serial crystallography data?
Temperature vs global and site-specific radiation damage ---
RT serial crystallography
Temperature vs multiple
conformers
Thanks,
Minmin
> Dear James,
>
> 10) are the biological questions that you can answer with a
(crystal)
> structure sufficiently relevant to justify the resources?
>
> Best,
> Tim
>
>
>
> Am 15.07.2019 21:44, schrieb Holton, James M:
>> Hello folks,
>>
>> I have the distinct honor of chairing the next Gordon Research
>> Conference on Diffraction Methods in Structural Biology (July
26-31
>> 2020). This meeting will focus on the biggest
challenges currently
>> faced by structural biologists, and I mean actual real-world
>> challenges. As much as possible, these challenges
will take the form
>> of
>> friendly competitions with defined parameters, data, a scoring
system,
>> and "winners", to be established along with other
unpublished results
>> only at the meeting, as is tradition at GRCs.
>>
>> But what are the principle challenges in biological structure
>> determination today? I of course have my own ideas,
but I feel like
>> I'm
>> forgetting something. Obvious choices are:
>> 1) getting crystals to diffract better
>> 2) building models into low-resolution maps (after failing at
#1)
>> 3) telling if a ligand is really there or not
>> 4) the phase problem (dealing with weak signal, twinning and
>> pseudotranslation)
>> 5) what does "resolution" really mean?
>> 6) why are macromolecular R factors so much higher than
small-molecule
>> ones?
>> 7) what is the best way to process serial crystallography
data?
>> 8) how should one deal with non-isomorphism in multi-crystal
methods?
>> 9) what is the "structure" of something that won't sit
still?
>>
>> What am I missing? Is industry facing different
problems than
>> academics? Are there specific challenges facing
electron-based
>> techniques? If so, could the combined strength of
all the world's
>> methods developers solve them? I'm interested in
hearing the voice of
>> this community. On or off-list is fine.
>>
>> -James Holton
>> MAD Scientist
>>
>>
>>
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>
> --
> --
> Tim Gruene
> Head of the Centre for X-ray Structure Analysis
> Faculty of Chemistry
> University of Vienna
>
> Phone: +43-1-4277-70202
>
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>
>
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