Sergei, That depends heavily on the protein, so I don't know if a statistical study will accurately reflect the importance of temperature. With some proteins, no matter how thoroughly you sample chemical space, temperature is critical, and a study would have to focus on such a protein. The data available is probably biased, because it is far easier to do crystallization experiments close to room temperature, and most proteins for which structures have actually been determined probably were not crystallized at 4 degrees C. I would venture to guess that there is a large protein structure space that has yet to be explored comprising proteins that don't normally form highly ordered crystals close to room temperature (at least in their native states, i.e., without creative protein redesigns coupling the structure of interest to an additional subunit or domain that facilitates crystallization at higher temperatures).
Daniel M. Himmel, Ph. D. E-mail : danielmhim...@gmail.com URL : http://www.DanielMhimmel.com/index_Xtal.html <http://www.danielmhimmel.com/index_Xtal.html> On Thu, Aug 1, 2019 at 5:24 AM Sergei Strelkov <sergei.strel...@kuleuven.be> wrote: > Dear all, > > > I wondered if someone could point me to a recent study on the importance > of temperature during initial search for crystallization conditions. It > would be interesting to see any real statistics on this subject. > > > We typically try to perform screening at at two temperatures, such as > duplicating a given kit screen at 20C and 4C if there is enough sample. My > 'gut feeling' is that this is not as important as sampling the chemical > space though. > > > Thank you! > > Sergei > > > Prof. Sergei V. Strelkov Laboratory for Biocrystallography Department of > Pharmaceutical Sciences, KU Leuven O&N2, Campus Gasthuisberg, Herestraat 49 > bus 822, 3000 Leuven, Belgium Phone: +32 16 33 08 45, mobile: +32 486 29 41 > 32 Lab pages: http://pharm.kuleuven.be/Biocrystallography > <http://pharm.kuleuven.be/anafar> > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
