Re: [ccp4bb] Better Beamline suggestion!

[Wim Burmeister]

Hello, the problem can rather be due to mechanical deformations of the crystal upon mounting and freezing (bent needle, for example). If the crystals are needle shaped, aligning the long axis with the spindle axis and using a beamline with a very small beamsize "microfocus" and a helical scan in order to expose a sufficient volume will help. Best Wim On 19/08/2019 17:08, Chandramohan Kattamuri wrote: Dear All We recently collected a data set at APS, Chicago with unit cell dimensions of 68.4; 68.4 and 991.6 A. Our diffraction data extends to 3A with the APS set up, however, the long axis has been problematic, resulting in streaking of the diffraction data and requires a very specific orientation of the crystal for usable diffraction. Can anyone recommend beamlines that can give us higher resolution, or a source with a better goniometer allowing for more angle manipulation after looping? Thank a lot Chandra K To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 -- Wim Burmeister Professeur Institut de Biologie Structurale (IBS) CIBB 71 avenue des Martyrs / CS 20192 38044 Grenoble Cedex 9, FRANCE E-mail: wim.burmeis...@ibs.fr Tel:    +33 (0) 457 42 87 41       Fax: +33 (0) 476 20 94 00 website                                    map Changement climatique : «Les autres combats n’ont aucun sens si celui-là est perdu» (Aurélien Barrau)            To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1