Why do you think you are rejecting anomalous data? What do the normal tell-tales reveal, like anom CC?
JPK +++++++++++++++++++++++++++++++++++++++++++++++++ Jacob Pearson Keller Research Scientist / Looger Lab HHMI Janelia Research Campus 19700 Helix Dr, Ashburn, VA 20147 Desk: (571)209-4000 x3159 Cell: (301)592-7004 +++++++++++++++++++++++++++++++++++++++++++++++++ The content of this email is confidential and intended for the recipient specified in message only. It is strictly forbidden to share any part of this message with any third party, without a written consent of the sender. If you received this message by mistake, please reply to this message and follow with its deletion, so that we can ensure such a mistake does not occur in the future. -----Original Message----- From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> On Behalf Of L. Doyle Sent: Monday, August 26, 2019 6:45 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] SeMet data I have some Seleno-Methionine protein crystals (12 SeMet of 211 amino acids, incorporation verified by Mass Spec). I've already collected several datasets (ALS BL5.0.2) but I seem to be losing (rejecting?) a lot of anomalous signal during data processing. I'm most familiar with HKL2000, but I have tried XDS and DIALS auto-processing. Here is a scan: https://urldefense.proofpoint.com/v2/url?u=https-3A__ibb.co_LZqm33p&d=DwIFaQ&c=LU6cRtx0xgB8s29tIz9Olw&r=eLCg9eJ4Rs_LnxfUWsp7FSxhIEcZYmTSU4Uyq1bRYPI&m=IY2dWiiG1i1JgjTXPAPNw3-KHrUr53w37DPc7mNTmQk&s=eA1o_iIowAlvk0KtH7k81LUBeSWfBfCsL7yvZK7KWvM&e= and here is an example of a frame: https://urldefense.proofpoint.com/v2/url?u=https-3A__ibb.co_gR3ZR47&d=DwIFaQ&c=LU6cRtx0xgB8s29tIz9Olw&r=eLCg9eJ4Rs_LnxfUWsp7FSxhIEcZYmTSU4Uyq1bRYPI&m=IY2dWiiG1i1JgjTXPAPNw3-KHrUr53w37DPc7mNTmQk&s=0Vn8zTPkITbHZuSG2lgAXAZZ5rpwTmNDfRvUjYPvYXs&e= . Each frame is 0.25° and I'm using inverse beam with wedge size 1°. Maybe I need to adjust my collection strategy? All previous datasets have been in space group P 21 with dimensions of approx. 24.5Å, 85Å, 40Å, 90°, 96.5°, 90°. I'm sure there are additional things I can be doing in HKL but I've run out of ideas. Any advice or recommendations would be appreciated. Please let me know if you need additional information. Thank you, Lindsey ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwIFaQ&c=LU6cRtx0xgB8s29tIz9Olw&r=eLCg9eJ4Rs_LnxfUWsp7FSxhIEcZYmTSU4Uyq1bRYPI&m=IY2dWiiG1i1JgjTXPAPNw3-KHrUr53w37DPc7mNTmQk&s=5TnOlPBXoRYKCMMRN52SE7gbBgtV_HHFJI-CpkNUf84&e= ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
