Hi, that's typical behaviour for an elongated/disordered molecule, given that SEC separates based on hydrodynamic radius, not MW. Petri
Petri Kursula ---------- Professor ---------- Department of Biomedicine University of Bergen, Norway http://www.uib.no/en/rg/petrikursula <http://www.uib.no/en/persons/Petri.Kursula> petri.kurs...@uib.no <mailto:petri.kurs...@biomed.uib.no> ---------- Faculty of Biochemistry and Molecular Medicine University of Oulu, Finland ---------- > On 27 Aug 2019, at 07:57, Natesh Ramanathan <nat...@iisertvm.ac.in> wrote: > > Dear Friends, > > Can you share your experience with examples of MALS giving lower > molecular weight (Eg. Monomer) and SEC giving higher molecular weight (Eg. > Dimer), for the same protein sample? > > If you have/know any published paper, can you please point me to that > reference paper or send me the paper? > > Many thanks. > Best regards, > Natesh > > > -- > ---------------------------------------------------------- > "Live Simply and do Serious Things .. " > - Dorothy Mary Crowfoot Hodgkin OM, FRS > > "In Science truth always wins" > - Max Ferdinand Perutz OM FRS > ---------------------------------------------------------- > Dr. Ramanathan Natesh > Assistant Professor, > School of Biology, > Indian Institute of Science Education and Research Thiruvananthapuram > (IISER-TVM), > Maruthamala P.O., Vithura, > Thiruvananthapuram, 695551, Kerala, India > > nat...@iisertvm.ac.in <mailto:nat...@iisertvm.ac.in> > http://www.researcherid.com/rid/C-4488-2008 > <http://www.researcherid.com/rid/C-4488-2008> > ORCID: http://orcid.org/0000-0002-1145-5962 > <http://orcid.org/0000-0002-1145-5962> > https://publons.com/author/1520837/ramanathan-natesh#profile > <https://publons.com/author/1520837/ramanathan-natesh#profile> > http://faculty.iisertvm.ac.in/natesh <http://faculty.iisertvm.ac.in/natesh> > > Office Ph. 0091- 471-2778087 > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > <https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
