Hi Sylvia, A screen specifically designed to crystallize protein-DNA complexes is described in this paper: https://www.ncbi.nlm.nih.gov/pubmed/22869139 <https://www.ncbi.nlm.nih.gov/pubmed/22869139>
Back when it was just published, I manually prepared the 48 conditions and it was not too difficult (each one is a combination of one buffer, one or two salts, and one PEG). Now you can buy it pre-made from at least one supplier (possibly available elsewhere too, I haven’t checked): https://www.kerafast.com/product/287/protein-nucleic-acid-complex-crystal-screen <https://www.kerafast.com/product/287/protein-nucleic-acid-complex-crystal-screen> Good luck with your project! Guillaume > On Oct 15, 2019, at 01:48, Sylvia Fanucchi <sylvia.fanuc...@wits.ac.za> wrote: > > > Hi > Can anyone recommend a good crystal screen to use to crystalise protein-NA > complexes? > > Kind Regards > Sylvia > This communication is intended for the addressee only. It is confidential. If > you have received this communication in error, please notify us immediately > and destroy the original message. You may not copy or disseminate this > communication without the permission of the University. Only authorised > signatories are competent to enter into agreements on behalf of the > University and recipients are thus advised that the content of this message > may not be legally binding on the University and may contain the personal > views and opinions of the author, which are not necessarily the views and > opinions of The University of the Witwatersrand, Johannesburg. All agreements > between the University and outsiders are subject to South African Law unless > the University agrees in writing to the contrary. > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > <https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
